Project Type MQP Submission date 2006-01-04 Author Christopher S Parker, BIO URN E-project-010406-095229 Sponsor University of Massachusetts Medical School Title Comparison of Codon Optimized and Non-Optimized Influenza HA DNA Vaccines Advisor Adams, David S., BB Availability unrestricted Abstract
An effective antibody response is essential for immunity against influenza (Flu) virus infection and is the primary goal for Flu vaccines. In this study, codon optimized and wild type DNA vaccines expressing hemmaglutinin (HA) antigens of human flu viruses A/H1N1/NewCal/20/99 (H1 serotype) were compared. Expression levels of HA antigens were increased in 293T cells transiently transfected with codon optimized as compare to the wild type HA gene construct. The codon optimized vaccine was able to elicit a greater immune response in rabbits and mice as measured by ELISA testing of the temporal response and end antibody titers.
Files ChrisParkerMQP.pdf
Browse by Author | Browse by Department | Search all available E-projects
![[WPI]](/Buttons/seal.gif){kind=small}
![[Library]](/Academics/Library/Buttons/lib.gif){kind=small}
![[Home]](/Buttons/home.gif){kind=small}
![[Top]](/Buttons/top.gif){kind=small}