Title page for E-project-010908-142942

Project Type MQP

Submission date 2008-01-09

Author
Martha R Addorio, BIO

URN E-project-010908-142942

Sponsor University of Massachusetts Medical School

Title PERICENTRIN KNOCKDOWN BY RNAi DISRUPTS INSULIN SECRETION IN CULTURED MURINE INSULINOMA CELLS

Advisor
Adams, David S., BB

Availability unrestricted

Abstract
The centrosome functions in spindle formation, transport of cytoplasmic vesicles, and regulation of cell structure. Pericentrin, a centrosomal protein, was chosen to study for its role in microtubule nucleation and organization, cell cycle progression and ciliogenesis. This project's purpose was to determine if the amount of endogenous pericentrin would affect insulin storage or secretion in pancreatic beta cells. Immunofluorescent staining and electron microscopy revealed that pericentrin co-localizes to the granules of beta cells. After Pericentrin depletion with RNAi, a lower number of mature insulin granules was found, and a high amount of insulin was secreted into the media. This dys-regulation of insulin secretion was also verified by in vivo experiments.

Files
MarthaAddorioMQPFinal.pdf

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Project Type	MQP
Submission date	2008-01-09
Author	Martha R Addorio, BIO
URN	E-project-010908-142942
Sponsor	University of Massachusetts Medical School
Title	PERICENTRIN KNOCKDOWN BY RNAi DISRUPTS INSULIN SECRETION IN CULTURED MURINE INSULINOMA CELLS
Advisor	Adams, David S., BB
Availability	unrestricted
Abstract The centrosome functions in spindle formation, transport of cytoplasmic vesicles, and regulation of cell structure. Pericentrin, a centrosomal protein, was chosen to study for its role in microtubule nucleation and organization, cell cycle progression and ciliogenesis. This project's purpose was to determine if the amount of endogenous pericentrin would affect insulin storage or secretion in pancreatic beta cells. Immunofluorescent staining and electron microscopy revealed that pericentrin co-localizes to the granules of beta cells. After Pericentrin depletion with RNAi, a lower number of mature insulin granules was found, and a high amount of insulin was secreted into the media. This dys-regulation of insulin secretion was also verified by in vivo experiments.
Files	MarthaAddorioMQPFinal.pdf