Worcester Polytechnic Institute Electronic Projects Collection

Title page for E-project-031209-205737


Project TypeMQP
Submission date2009-03-12
Authors
  • Rebecca R Alan, BIO
  • Laura J Belen, BIO
  • URNE-project-031209-205737
    TitleA New Assay for Quantifying Pentosidine Levels in Avian Species
    Advisor
  • Rulfs, Jill, BB
  • Availability unrestricted

    Abstract

    The assay used in previous studies to quantify pentosidine levels was too costly and time consuming to be considered practical. The goal of our study was to develop a new assay to determine pentosidine concentration in avian species. Skin samples were harvested from loons of known/unknown age as well as from five

    roosters that were all five months of age. It was discovered that pentosidine concentration could be determined by chloroform methanol delipidation, collagen extraction by RIPA buffer, and analysis by fluorometry. In the future, it is hoped that this assay will aid in research to determine the age of wild birds admitted to wildlife clinics for treatment and necropsy.

    Files
  • Final_MQP.pdf

    There is one file which has been withheld at the advisor's request.


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