Title page for E-project-031405-161010

Project Type MQP

Submission date 2005-03-14

Author
Alexia T Kedves, BIO

URN E-project-031405-161010

Sponsor Abbott BASF

Title Analysis of Matrix Attachment Region in an Experimental Vector, pA205

Advisor
Adams, David S., BB

Availability unrestricted

Abstract
Recent literature has shown that Matrix and Scaffold Attachment Regions (MARs or SARs) are DNA domains that enhance transcriptional frequency and increase gene expression levels in cells. Research has shown that cell lines containing MARs or SARs are more stable and more productive. Researchers at Abbott Bioresearch Center in Worcester, MA have found two possible MAR regions in an antibody producing cell line. In order to determine whether these regions function as MARs, and if the observed high levels of expression are specific to this cell line, the sequences in question were inserted upstream of the reporter gene, Enhanced Green Fluorescent Protein (EGFP) and tested using FACS analysis. The data indicated the sequences under investigation indeed increased expression of EGFP in stable cell lines, indicating that these sequences can be used to improve transfection and establish high expressing, stable CHO cell lines.

Files
MQP.pdf

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Project Type	MQP
Submission date	2005-03-14
Author	Alexia T Kedves, BIO
URN	E-project-031405-161010
Sponsor	Abbott BASF
Title	Analysis of Matrix Attachment Region in an Experimental Vector, pA205
Advisor	Adams, David S., BB
Availability	unrestricted
Abstract Recent literature has shown that Matrix and Scaffold Attachment Regions (MARs or SARs) are DNA domains that enhance transcriptional frequency and increase gene expression levels in cells. Research has shown that cell lines containing MARs or SARs are more stable and more productive. Researchers at Abbott Bioresearch Center in Worcester, MA have found two possible MAR regions in an antibody producing cell line. In order to determine whether these regions function as MARs, and if the observed high levels of expression are specific to this cell line, the sequences in question were inserted upstream of the reporter gene, Enhanced Green Fluorescent Protein (EGFP) and tested using FACS analysis. The data indicated the sequences under investigation indeed increased expression of EGFP in stable cell lines, indicating that these sequences can be used to improve transfection and establish high expressing, stable CHO cell lines.
Files	MQP.pdf