Title page for E-project-042408-123650

Project Type MQP

Submission date 2008-04-24

Authors
Danielle Dionne, BIO
Stacy Ann Krzyzewski, BIO

URN E-project-042408-123650

Title Ependymin Immunoreactivity in Limulus polyphemus

Advisor
Gibson, Daniel G, BB

Availability unrestricted

Abstract
Ependymin (EPN) is a neurotrophic factor discovered in memory consolidation in goldfish. Sections of the EPN gene sequences have since been found in many other species, including the horseshoe crab. Previous research suggests that EPN may be instrumental in nerve growth. Using a new antibody against the bioactive 8 aa fragment of EPN, we have shown that EPN is present in the central and peripheral nervous system of developing horseshoe crabs. It was evident in the interstices between axons of regenerating limbs. HRP staining and fluorescence microscopy were used to observe EPN immunoreactivity. TEM and Immunogold staining were also employed to further support our results from fluorescence and light microscopy techniques and give us a more detailed view on EPN's mechanism of action.

Files
EpendyminImmunoreactivityinLimuluspolyphemus.pdf

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Project Type	MQP
Submission date	2008-04-24
Authors	Danielle Dionne, BIO Stacy Ann Krzyzewski, BIO
URN	E-project-042408-123650
Title	Ependymin Immunoreactivity in Limulus polyphemus
Advisor	Gibson, Daniel G, BB
Availability	unrestricted
Abstract Ependymin (EPN) is a neurotrophic factor discovered in memory consolidation in goldfish. Sections of the EPN gene sequences have since been found in many other species, including the horseshoe crab. Previous research suggests that EPN may be instrumental in nerve growth. Using a new antibody against the bioactive 8 aa fragment of EPN, we have shown that EPN is present in the central and peripheral nervous system of developing horseshoe crabs. It was evident in the interstices between axons of regenerating limbs. HRP staining and fluorescence microscopy were used to observe EPN immunoreactivity. TEM and Immunogold staining were also employed to further support our results from fluorescence and light microscopy techniques and give us a more detailed view on EPN's mechanism of action.
Files	EpendyminImmunoreactivityinLimuluspolyphemus.pdf