Project Type MQP Submission date 2009-04-24 Author Tommy F Tashjian, BIO URN E-project-042409-193454 Title Creation, Induction, and Purification of His-Tagged and FLAG-Tagged OXR2 Advisor Adams, David S., BB Availability unrestricted Abstract
Reactive oxygen species (ROS) are reduced forms of oxygen created as byproducts of cellular metabolic processes. ROS cause oxidative damage to many organic molecules within the cell, especially to DNA. OXR2 provides cells with protection against this damage, but its biochemical and functional properties have not yet been studied in depth. The purpose of this project was to create and purify a His-tagged version of human OXR2 and to induce a FLAG-tagged
OXR2 in E. coli. These tagged proteins will be used in studies of this protein and its mechanism.
Files MQP_Tommy_Tashjian.pdf
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