Project Type MQP Submission date 2005-04-25 Author Erich A Lidstone, BC URN E-project-042505-141611 Sponsor Rita Bortell, PhD, UMASS Medical School Title The Role of Beta-Cell Apoptosis in Diabetes Pathogenesis in Biobreeding/Worcester Diabetes Prone Rats Advisor Adams, David S., BB Availability unrestricted Abstract
The purpose of this project was to correlate the time of onset of pancreatic a-cell apoptosis with the onset of diabetes in the BioBreeding Diabetes Prone (BB/DP) rat model to provide an initial determination of whether apoptosis is a primary or secondary event in the disease onset. Immunofluorescence microscopy was used to assay the appearance of Annexin-V apoptotic protein marker and the appearance of DNA fragmentation (via TUNEL assays). Due to time constraints, confirmation of beta-cell apoptosis was not possible in this study; future research, however, should conclusively prove the role of beta-cell apoptosis in diabetes onset.
Files EAL-BC-05-MQP.pdf
Browse by Author | Browse by Department | Search all available E-projects
![[WPI]](/Buttons/seal.gif){kind=small}
![[Library]](/Academics/Library/Buttons/lib.gif){kind=small}
![[Home]](/Buttons/home.gif){kind=small}
![[Top]](/Buttons/top.gif){kind=small}