Title page for E-project-042507-154131

Project Type MQP

Submission date 2007-04-25

Author
James Citrone, BC

URN E-project-042507-154131

Title Approaches to Localizing Small RNAs and Proteins in the Cell

Advisor
Wobbe, Kristin K, CH

Availability unrestricted

Abstract
This project focused on the localization of small RNAs as well as certain proteins in mammalian cells grown in culture. Immunofluorescence and confocal microscopy were used to locate signal recognition particle (SRP) RNA and three associated protein subunits, and also telomerase RNA and two associated proteins. Several different cell types were used to study the molecules, as well as multiple techniques for locating these molecules including fluorescence in situ nucleic acid hybridization, immunostaining, and fluorescent protein gene transfection and expression.

Files
JamesCitroneFinalMQP.pdf

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Project Type	MQP
Submission date	2007-04-25
Author	James Citrone, BC
URN	E-project-042507-154131
Title	Approaches to Localizing Small RNAs and Proteins in the Cell
Advisor	Wobbe, Kristin K, CH
Availability	unrestricted
Abstract This project focused on the localization of small RNAs as well as certain proteins in mammalian cells grown in culture. Immunofluorescence and confocal microscopy were used to locate signal recognition particle (SRP) RNA and three associated protein subunits, and also telomerase RNA and two associated proteins. Several different cell types were used to study the molecules, as well as multiple techniques for locating these molecules including fluorescence in situ nucleic acid hybridization, immunostaining, and fluorescent protein gene transfection and expression.
Files	JamesCitroneFinalMQP.pdf