Title page for E-project-042507-185231

Project Type MQP

Submission date 2007-04-25

Author
Alissa M. Paquette, BIO

URN E-project-042507-185231

Sponsor Patrick Emery-Le, Ph.D.

Title Circadian Responses to Temperature Cycles in Cryb mutant Drosophila

Advisor
Adams, David S., BB

Availability unrestricted

Abstract
In Drosophila melanogaster circadian rhythms can be entrained by light and temperature. Three essential proteins for the Drosophila circadian clock are PER, TIM, and the photoreceptor CRY. In cryb mutant flies that lack CRY, PER and TIM do not cycle under light/dark conditions. A hypothesis was made that PER and TIM would oscillate normally in a temperature cycle under constant darkness in cryb flies. Through immunoblotting it was found that PER and TIM oscillate in cryb flies similarly to that of wt, supporting the idea that CRY is only required for light responses. Behavioral tests were performed to see if adding a temperature cycle into a LD cycle would cause cryb fly activity to shift. Cryb flies showed greater sensitivity to temperature cycles than wt flies under these conditions.

Files
AlissaPaquetteMQP.pdf

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Project Type	MQP
Submission date	2007-04-25
Author	Alissa M. Paquette, BIO
URN	E-project-042507-185231
Sponsor	Patrick Emery-Le, Ph.D.
Title	Circadian Responses to Temperature Cycles in Cryb mutant Drosophila
Advisor	Adams, David S., BB
Availability	unrestricted
Abstract In Drosophila melanogaster circadian rhythms can be entrained by light and temperature. Three essential proteins for the Drosophila circadian clock are PER, TIM, and the photoreceptor CRY. In cryb mutant flies that lack CRY, PER and TIM do not cycle under light/dark conditions. A hypothesis was made that PER and TIM would oscillate normally in a temperature cycle under constant darkness in cryb flies. Through immunoblotting it was found that PER and TIM oscillate in cryb flies similarly to that of wt, supporting the idea that CRY is only required for light responses. Behavioral tests were performed to see if adding a temperature cycle into a LD cycle would cause cryb fly activity to shift. Cryb flies showed greater sensitivity to temperature cycles than wt flies under these conditions.
Files	AlissaPaquetteMQP.pdf