Title page for E-project-042508-125134

Project Type MQP

Submission date 2008-04-25

Authors
Katarzyna Koscielska, BC
Oliver Salmon, BC

URN E-project-042508-125134

Title Mapping of HTLV-1 Tax Functional Domain Conferring Cellular Senescence

Advisors
Heilman, Destin, CH
Wobbe, Kristin K, BC

Availability unrestricted

Abstract
Tax is a human T-lymphotrophic virus type 1 (HTLV-1) protein that predisposes transformation of certain cell types by inducing cellular senescence. To map the Tax domain that confers this senescence, PCR-based truncation mutagenesis was used. PCR products included full length tax and mutants lacking the C-terminal quarter, C-terminal half, and N-terminal quarter of the protein. These mutants and the wild type were cloned into a yeast plasmid vector under the control of a GAL1 promoter and used to transform Y187 S. cerevisiae. After Trp- selection and galactose induction, the transformed yeast phenotypes were observed. Based on comparison of wild type and truncation phenotypes, it was concluded that the senescence-conferring domain could be located in the C-terminal region of the protein.

Files
BCH-MQP-Report.pdf

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Project Type	MQP
Submission date	2008-04-25
Authors	Katarzyna Koscielska, BC Oliver Salmon, BC
URN	E-project-042508-125134
Title	Mapping of HTLV-1 Tax Functional Domain Conferring Cellular Senescence
Advisors	Heilman, Destin, CH Wobbe, Kristin K, BC
Availability	unrestricted
Abstract Tax is a human T-lymphotrophic virus type 1 (HTLV-1) protein that predisposes transformation of certain cell types by inducing cellular senescence. To map the Tax domain that confers this senescence, PCR-based truncation mutagenesis was used. PCR products included full length tax and mutants lacking the C-terminal quarter, C-terminal half, and N-terminal quarter of the protein. These mutants and the wild type were cloned into a yeast plasmid vector under the control of a GAL1 promoter and used to transform Y187 S. cerevisiae. After Trp- selection and galactose induction, the transformed yeast phenotypes were observed. Based on comparison of wild type and truncation phenotypes, it was concluded that the senescence-conferring domain could be located in the C-terminal region of the protein.
Files	BCH-MQP-Report.pdf