Title page for E-project-042605-180407

Project Type MQP

Submission date 2005-04-26

Authors
Jaime Lynn Grouf, BIO
Stacia Lee Richards, BIO

URN E-project-042605-180407

Sponsor Paul D. Gardner, Ph.D

Title Real-time PCR Study of alpha4-beta2 nAChR Gene Expression During Chronic Nicotine Treatment of H69 Cells

Advisor
Adams, David S., BB

Availability unrestricted

Abstract
Previous studies using reverse transcriptase PCR have shown increased nAChR receptor subunit expression with nicotine treatment. We proposed to treat the H69 human lung carcinoma cell line with nicotine and observe the effects on gene expression using real-time PCR with the hypothesis that nicotine treatment alters gene expression of alpha4-alpha2 subunits. In analyzing a 4 day 100 M nicotine treatment of the H69 cell line, a significant increase in gene expression of the alpha4 subunit was observed after 24 hours.

Files
MQP.pdf

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Project Type	MQP
Submission date	2005-04-26
Authors	Jaime Lynn Grouf, BIO Stacia Lee Richards, BIO
URN	E-project-042605-180407
Sponsor	Paul D. Gardner, Ph.D
Title	Real-time PCR Study of alpha4-beta2 nAChR Gene Expression During Chronic Nicotine Treatment of H69 Cells
Advisor	Adams, David S., BB
Availability	unrestricted
Abstract Previous studies using reverse transcriptase PCR have shown increased nAChR receptor subunit expression with nicotine treatment. We proposed to treat the H69 human lung carcinoma cell line with nicotine and observe the effects on gene expression using real-time PCR with the hypothesis that nicotine treatment alters gene expression of alpha4-alpha2 subunits. In analyzing a 4 day 100 M nicotine treatment of the H69 cell line, a significant increase in gene expression of the alpha4 subunit was observed after 24 hours.
Files	MQP.pdf