Project Type MQP Submission date 2005-04-26 Author Roland W Smith, BBI URN E-project-042605-194234 Center Worcester, MA Sponsor Dr. Ennis, UMass Title Generation of a Recombinant Vaccinia Virus Expressing the New H5 Subtype of the Influenza A Hemmagglutinin Gene Advisor Adams, David S., BB Availability unrestricted Abstract
This project was designed to generate a recombinant vaccinia virus expressing the new H5 subtype of the HA gene as part of eventually defining the CTL epitope on the avian HA protein recognized by this human T cell clone. The result was achieved by first generating a transfer vector for homologous recombination. The vector was used to generate a recombinant vaccinia virus expressing the HA gene. Expression of the HA gene product was confirmed at both transcriptional and translational levels, using RT-PCR and immunoprecipitations, respectively.
Files 29MAR05MQPRSmith.pdf
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