Title page for E-project-042606-192358

Project Type MQP

Submission date 2006-04-26

Author
Elisabeth A Janiszewski, BC

URN E-project-042606-192358

Title Petrubation Analysis of a Potassium Channel Beta Subunit

Advisor
Wobbe, Kristin K, CH

Availability unrestricted

Abstract
KCNQ1 is a voltage-gated potassium channel that co-assembles with the KCNE family of transmembrane peptides. KCNQ1/KCNE3 channels are found in colonic crypt and airway epithelial cells and are constitutively conducting at normal resting potentials. Using site-directed mutagenesis, residues 87-96 in the C-terminus were mutated to alanine. The RNA of these mutants was microinjected into Xenopus oocytes and examined using a two-electrode voltage clamp. Results were compared to KCNE1 to determine the role of C-terminus in KCNQ1 modulation.

Files
Janiszewski-MQP.pdf

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Project Type	MQP
Submission date	2006-04-26
Author	Elisabeth A Janiszewski, BC
URN	E-project-042606-192358
Title	Petrubation Analysis of a Potassium Channel Beta Subunit
Advisor	Wobbe, Kristin K, CH
Availability	unrestricted
Abstract KCNQ1 is a voltage-gated potassium channel that co-assembles with the KCNE family of transmembrane peptides. KCNQ1/KCNE3 channels are found in colonic crypt and airway epithelial cells and are constitutively conducting at normal resting potentials. Using site-directed mutagenesis, residues 87-96 in the C-terminus were mutated to alanine. The RNA of these mutants was microinjected into Xenopus oocytes and examined using a two-electrode voltage clamp. Results were compared to KCNE1 to determine the role of C-terminus in KCNQ1 modulation.
Files	Janiszewski-MQP.pdf