Title page for E-project-042612-132936

Project Type	MQP
Submission date	2012-04-26
Authors	Alec E Morrison, BC Diego Prentice-Webb, BC
URN	E-project-042612-132936
Title	Protein Interactions of the Porcine Circovirus Viral Protein 3
Advisor	Heilman, Destin, CH
Availability	restricted
Abstract The porcine circovirus type 2 viral protein 3 (VP3) and its homolog, the chicken anemia virus protein Apoptin, have been shown to induce p53 independent apoptosis in transformed cells. The present study investigated VP3’s ability to multimerize and associate with the anaphase promoting complex/cylcosome’s (APC/C) subunit 1 similar to Apoptin. A novel FRET technique involving GFP and DsRed fluorophores was successful in demonstrating Apoptin multimerization and provides way to explore VP3’s multimeric capability. In addition, FLAG-tagged VP3 2A and 2B constructs were created for future immunoprecipitation studies of VP3 multimerization and association with the APC/C subunit 1. Understanding such mechanisms may have implications for VP3's candidacy as a cancer therapeutic.
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