Project Type MQP Submission date 2012-04-26 Authors Tom Clinckemaillie, BE Franco Antonio Oshiro, BE Joseph Michael Worthy, BE URN E-project-042612-144641 Title Automated Cardiomyocyte Isolation Advisor Gaudette, Glenn R., BE Availability unrestricted
Myocardial infarction is characterized by the death of cardiomyocytes, the fundamental contractile units of the heart. Due to the cardiomyocyte's physiological importance, a more efficient and economical alternative to manual cell isolation is needed to obtain cells for in vitro studies. We designed a device that automates the teasing and mechanical agitation of heart tissue to obtain isolated cardiomyocytes. Through testing, our device met design goals for cell yield and viability while greatly reducing overall isolation duration and user interaction.
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