
Project Type MQP Submission date 2006-04-27 Authors Zachary R Gautreau, BE Jeremy Ryan Griffin, BE Todd F Peterson, PH Ploypan Thongpradit, BE URN E-project-042706-113033 Title Characterizing Viscoelastic Properties of Polyacrylamide Gels Advisors Billiar, Kristen, BE Burnham, Nancy A., PH Availability unrestricted Abstract
Despite its common use in cell culture studies, polyacrylamide gel's mechanical properties remain unclear. To characterize this gel fully, atomic force microscopy, uniaxial testing, rheology, and ball indentation were used. The range of the elastic moduli was 1.9-7.5 kPa and 18.1-39.4 kPa, for 5%/0.025% and 8%/0.08% gel concentrations, respectively, and a Poisson's ratio of 0.45 was found. Future research utilizing polyacrylamide as a cell culture substrate should indicate how the gel was characterized and acknowledge the uncertainty in stiffness values.
Files Polyacrylamide_MQP_Final_Report.pdf
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