Title page for E-project-042706-123031

Project Type	MQP
Submission date	2006-04-27
Author	William Michael Flaherty, BC
URN	E-project-042706-123031
Title	Analysis of QK-1 Binding to mRNA Targets Implicated in the Schizophrenia Pathway
Advisor	Adams, David S., BB
Availability	unrestricted
Abstract Down regulation of QK-1 correlates with reduced levels of SFRS5,hnRNP A1, NCAM, hnRNP D, and SRRM 1RNA. QK-1 is an RNA binding protein that may regulate the localization and stability of these mRNA transcripts. To test if QK-1 can bind to these candidate targets, quantitative binding experiments were performed using fluorescence polarization, competition fluorescence polarization, and gel mobility shift assays. The data show that QK-1 binds with the highest affinity to hnRNP A1, NCAM and SRRM 1 transcripts, consistent with direct role for this protein in the regulation of these mRNAs. Interestingly, like QK-1, NCAM is also suspected to play a role in schizophrenia. The data show that QK-1 binds tightly to NCAM RNA, establishing a new link between these two schizophrenia candidates.
Files	MQP.pdf

Browse by Author | Browse by Department | Search all available E-projects