Title page for E-project-042706-145413

Project Type MQP

Submission date 2006-04-27

Authors
Sarah R Epstein, BIO
Ashley Renee Pyle, BIO
Agnieszka B Ziolek, BIO

URN E-project-042706-145413

Title Analysis of mig-10 expression to determine cell autonomy or nonautonomy in Caenorhabditis elegans

Advisor
Ryder, Elizabeth F, BB

Availability unrestricted

Abstract
Early in C. elegans development, several neurons must migrate to specific locations within the body. Mutations in mig-10 result in incomplete migration of several neurons, as well as defects in egg laying and excretory cell development. We tested the ability of each of the two mig-10 splice forms to rescue the egg laying defect; partial rescue was observed. These results indicate that perhaps more than one isoform of the MIG-10 protein must be expressed simultaneously in multiple cells in order to achieve total mutant rescue. To test the hypothesis that expression in specific neurons is required for rescue of the migration and excretory cell defects, constructs were made to express each mig-10 splice form in a particular neuron.

Files
ASA_MQP.pdf

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Project Type	MQP
Submission date	2006-04-27
Authors	Sarah R Epstein, BIO Ashley Renee Pyle, BIO Agnieszka B Ziolek, BIO
URN	E-project-042706-145413
Title	Analysis of mig-10 expression to determine cell autonomy or nonautonomy in Caenorhabditis elegans
Advisor	Ryder, Elizabeth F, BB
Availability	unrestricted
Abstract Early in C. elegans development, several neurons must migrate to specific locations within the body. Mutations in mig-10 result in incomplete migration of several neurons, as well as defects in egg laying and excretory cell development. We tested the ability of each of the two mig-10 splice forms to rescue the egg laying defect; partial rescue was observed. These results indicate that perhaps more than one isoform of the MIG-10 protein must be expressed simultaneously in multiple cells in order to achieve total mutant rescue. To test the hypothesis that expression in specific neurons is required for rescue of the migration and excretory cell defects, constructs were made to express each mig-10 splice form in a particular neuron.
Files	ASA_MQP.pdf