Title page for E-project-043009-084640

Project Type MQP

Submission date 2009-04-30

Author
Pramela T Nayak, BIO

URN E-project-043009-084640

Title The Expression of Ependymin in C. elegans Roundworms - II

Advisor
Gibson, Daniel G, BB

Availability unrestricted

Abstract
DNA and protein sequence homologies of ependymin, a neurotrophic factor of vertebrates, occur in the model organism Caenorhabditis elegans. We employed a new antibody against a bioreactive 8-aa ependymin fragment on C. elegans' four larval developmental stages, to verify its expression. This project utilized immunostaining in conjunction with HRP light, fluorescence, and transmission electron microscopies, to observe ependymin expression from the worm's early embryonic stage onwards to the L2 larval stage. This we witnessed most noticeably in developing embryos and along major nerve cords.

Files
PramelaNayakMQPCEL109Report.pdf

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Project Type	MQP
Submission date	2009-04-30
Author	Pramela T Nayak, BIO
URN	E-project-043009-084640
Title	The Expression of Ependymin in C. elegans Roundworms - II
Advisor	Gibson, Daniel G, BB
Availability	unrestricted
Abstract DNA and protein sequence homologies of ependymin, a neurotrophic factor of vertebrates, occur in the model organism Caenorhabditis elegans. We employed a new antibody against a bioreactive 8-aa ependymin fragment on C. elegans' four larval developmental stages, to verify its expression. This project utilized immunostaining in conjunction with HRP light, fluorescence, and transmission electron microscopies, to observe ependymin expression from the worm's early embryonic stage onwards to the L2 larval stage. This we witnessed most noticeably in developing embryos and along major nerve cords.
Files	PramelaNayakMQPCEL109Report.pdf