Title page for E-project-043009-101048

Project Type MQP

Submission date 2009-04-30

Authors
Shannon Brooks, BB
Sabrina L Zayas, BB

URN E-project-043009-101048

Sponsor Umass Medical

Title REGULATION OF SOX 4 AND SOX 11-MEDIATED GENE TRANSCRIPTION BY MIR-30-BASED RNAi

Advisor
Adams, David S., BB

Availability restricted

Abstract
This projects goal was to use a plasmid previously cloned in our laboratory encoding miR30 to determine its effectiveness to knock down the expression of Sox4 and Sox11, two genes closely associated with tumor initiation and progress. Western blots and luciferase reporter assays showed that miR30-based shRNAs resulted in strong knock-down of Sox4 and Sox11 expression and a decrease in their induction of Sox-mediated gene transcription in 3T3 cells. Our results suggest that miR30-based shRNAs might be used as an effective cellular tool to investigate the role of Sox4/11 in tumorigenesis.

Files
MQP.pdf
There is one file which has been withheld at the advisor's request.
indicates that a file or directory is accessible from the WPI campus network only.

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Project Type	MQP
Submission date	2009-04-30
Authors	Shannon Brooks, BB Sabrina L Zayas, BB
URN	E-project-043009-101048
Sponsor	Umass Medical
Title	REGULATION OF SOX 4 AND SOX 11-MEDIATED GENE TRANSCRIPTION BY MIR-30-BASED RNAi
Advisor	Adams, David S., BB
Availability	restricted
Abstract This projects goal was to use a plasmid previously cloned in our laboratory encoding miR30 to determine its effectiveness to knock down the expression of Sox4 and Sox11, two genes closely associated with tumor initiation and progress. Western blots and luciferase reporter assays showed that miR30-based shRNAs resulted in strong knock-down of Sox4 and Sox11 expression and a decrease in their induction of Sox-mediated gene transcription in 3T3 cells. Our results suggest that miR30-based shRNAs might be used as an effective cellular tool to investigate the role of Sox4/11 in tumorigenesis.
Files	MQP.pdf There is one file which has been withheld at the advisor's request. indicates that a file or directory is accessible from the WPI campus network only.