Title page for E-project-043009-105252

Project Type MQP

Submission date 2009-04-30

Authors
Shawn P. Carey, BE
Jonathan M. Charest, BE
Elizabeth C. Ellis, CM
Jason Z. Hu, BE

URN E-project-043009-105252

Title Design of a Co-culture System Using Collagen Microthreads to Facilitate Neovascularization

Advisors
Pins, George, BE
Rolle, Marsha W., BE

Availability restricted

Abstract
Several co-culture systems involving fibroblasts and endothelial cells have been used to characterize angiogenesis. However, these models have not been translated into therapies for chronic non-healing wounds. We developed an approach that may facilitate in vivo neovascularization and serve as an in vitro tool to study angiogenic mechanisms. Self-assembled collagen microthreads populated with human dermal fibroblasts (HDFs) were extruded using a novel method prior to surface seeding of human umbilical vein endothelial cells (HUVECs). Fluorescence microscopy confirmed the presence of both cell types after 48 hours of co-culture. These results demonstrate that a viable co-culture system can be established on collagen microthreads with these seeding methods.

Files
Microthread_MQP_2009.pdf
indicates that a file or directory is accessible from the WPI campus network only.

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Project Type	MQP
Submission date	2009-04-30
Authors	Shawn P. Carey, BE Jonathan M. Charest, BE Elizabeth C. Ellis, CM Jason Z. Hu, BE
URN	E-project-043009-105252
Title	Design of a Co-culture System Using Collagen Microthreads to Facilitate Neovascularization
Advisors	Pins, George, BE Rolle, Marsha W., BE
Availability	restricted
Abstract Several co-culture systems involving fibroblasts and endothelial cells have been used to characterize angiogenesis. However, these models have not been translated into therapies for chronic non-healing wounds. We developed an approach that may facilitate in vivo neovascularization and serve as an in vitro tool to study angiogenic mechanisms. Self-assembled collagen microthreads populated with human dermal fibroblasts (HDFs) were extruded using a novel method prior to surface seeding of human umbilical vein endothelial cells (HUVECs). Fluorescence microscopy confirmed the presence of both cell types after 48 hours of co-culture. These results demonstrate that a viable co-culture system can be established on collagen microthreads with these seeding methods.
Files	Microthread_MQP_2009.pdf indicates that a file or directory is accessible from the WPI campus network only.