Title page for E-project-043009-122007

Project Type MQP

Submission date 2009-04-30

Authors
Eric Morgan Evan-Browning, BC
McGhee Orme-Johnson, BC

URN E-project-043009-122007

Title Gene synthesis and expression of human torque teno virus VP3: Exploring the cancer-killing potential of an apoptin homolog

Advisor
Heilman, Destin, CH

Availability unrestricted

Abstract
Torque teno virus (TTV) is a small, non-enveloped animal virus that contains a single-stranded, circular DNA genome of 3.8 kb. Related to chicken anemia virus (CAV), TTV's ORF 3 may express a 105 residue protein which is homologous to CAV VP3 (apoptin)--a protein shown to multimerize and trigger apoptosis in cancer cells via a mechanism that involves nuclear translocation. Using gene synthesis, we generated the TTV ORF3 and expressed the gene fused to GFP. Here we demonstrate that TTV VP3 localizes to the cytoplasm of H1299 lung cancer cells and shows evidence of cytoplasmic aggregation. This aggregation is strikingly similar to CAV apoptin and may indicate a similar multimerization capacity for TTV VP3. However, an altered localization pattern may suggest a different mechanism of action.

Files
MQP_finaldraft.pdf

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Project Type	MQP
Submission date	2009-04-30
Authors	Eric Morgan Evan-Browning, BC McGhee Orme-Johnson, BC
URN	E-project-043009-122007
Title	Gene synthesis and expression of human torque teno virus VP3: Exploring the cancer-killing potential of an apoptin homolog
Advisor	Heilman, Destin, CH
Availability	unrestricted
Abstract Torque teno virus (TTV) is a small, non-enveloped animal virus that contains a single-stranded, circular DNA genome of 3.8 kb. Related to chicken anemia virus (CAV), TTV's ORF 3 may express a 105 residue protein which is homologous to CAV VP3 (apoptin)--a protein shown to multimerize and trigger apoptosis in cancer cells via a mechanism that involves nuclear translocation. Using gene synthesis, we generated the TTV ORF3 and expressed the gene fused to GFP. Here we demonstrate that TTV VP3 localizes to the cytoplasm of H1299 lung cancer cells and shows evidence of cytoplasmic aggregation. This aggregation is strikingly similar to CAV apoptin and may indicate a similar multimerization capacity for TTV VP3. However, an altered localization pattern may suggest a different mechanism of action.
Files	MQP_finaldraft.pdf