Title page for E-project-043009-134907

Project Type MQP

Submission date 2009-04-30

Authors
Allison Marie Lombardo, BB
Elaina Nichols, BC

URN E-project-043009-134907

Sponsor Umass Medical School

Title Toxicity and Anti-Tumor Effects of Cytarabine and Doxorubicin on Acute Myeloid Leukemia

Advisor
Adams, David S., BB

Availability unrestricted

Abstract
The goal of this project was to study the toxicity and anti-tumor effects of chemotherapy drugs Doxo and Ara-C as inhibitors of leukemic cells expressing the oncogene CBFB-MYH11, which causes AML. In vitro it was determined that Doxo and Ara-C affect the cell cycle of 3T3 cells, but have a lesser impact on ME-1 cells. In vivo WT mice can tolerate up to 10 doses of 6 mg/kg/every other day of Doxo, and 27 doses of 200 mg/kg/day of Ara-C. Leukemic mice treated with chemo drugs 4 weeks after transplantation survived 39 days with treatment, while mice treated 1 week after transplantation survived 19 days with treatment. Our research shows that two factors in decreasing toxicity and increasing efficacy of AML treatment are recovery time post irradiation and concentration of drug dose.

Files
Allison_and_Elaine_MQP_Final.pdf

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Project Type	MQP
Submission date	2009-04-30
Authors	Allison Marie Lombardo, BB Elaina Nichols, BC
URN	E-project-043009-134907
Sponsor	Umass Medical School
Title	Toxicity and Anti-Tumor Effects of Cytarabine and Doxorubicin on Acute Myeloid Leukemia
Advisor	Adams, David S., BB
Availability	unrestricted
Abstract The goal of this project was to study the toxicity and anti-tumor effects of chemotherapy drugs Doxo and Ara-C as inhibitors of leukemic cells expressing the oncogene CBFB-MYH11, which causes AML. In vitro it was determined that Doxo and Ara-C affect the cell cycle of 3T3 cells, but have a lesser impact on ME-1 cells. In vivo WT mice can tolerate up to 10 doses of 6 mg/kg/every other day of Doxo, and 27 doses of 200 mg/kg/day of Ara-C. Leukemic mice treated with chemo drugs 4 weeks after transplantation survived 39 days with treatment, while mice treated 1 week after transplantation survived 19 days with treatment. Our research shows that two factors in decreasing toxicity and increasing efficacy of AML treatment are recovery time post irradiation and concentration of drug dose.
Files	Allison_and_Elaine_MQP_Final.pdf