Title page for E-project-043009-150809

Project Type	MQP
Submission date	2009-04-30
Author	Alyson P Talbot, CH
URN	E-project-043009-150809
Sponsor	UMass Medical
Title	Potential Role of CTCF in Differential Papillomavirus Gene Expression
Advisor	Heilman, Destin, CH
Availability	unrestricted
Abstract Upon cellular differentiation the late genes of the Human Papillomavirus (HPV) are expressed. Recently, the CTCF DNA-binding protein has been found to be transcription factor and chromatin insulator. We examined if CTCF plays a role in viral transcription and late gene expression. Through ChIP analysis it was found that CTCF binds to two distinct regions of the Bovine Papillomavirus-1 genome. It was observed that the E2 protein and CTCF protein interact by co-immunoprecipitation. Using RNA interference, the effect of CTCF on viral transcription was examined. It was shown that E2 and L1 RNA levels are knocked out with lower levels of CTCF. Further studies should investigate if the type of interactions between E2 and CTCF, and the mechanism in which CTCF controls viral transcription.
Files	TalbotMQP.pdf

Browse by Author | Browse by Department | Search all available E-projects