Title page for E-project-043009-152556

Project Type MQP

Submission date 2009-04-30

Authors
Jessie Ann Brasefield, BC
Doug K Tischer, CH

URN E-project-043009-152556

Title Exploring Apoptin’s Anticancer Ability: Uncoupling Nuclear Export and Multimerization

Advisor
Heilman, Destin, CH

Availability unrestricted

Abstract
Apoptin, Chicken Anemia Virus VP3, selectively induces apoptosis in transformed cells but not primary cells. In all cases, Apoptin undergoes nucleocytoplasmic shuttling, but partitions into the nucleus of transformed cells and the cytoplasm of primary cells. The N-terminal NES of Apoptin and the domain for multimerization overlap and all previous attempts to uncouple these activities have failed. In this study, we successfully uncoupled nuclear export and multimerization through site-directed mutagenesis of ApI37A and ApI40A. Uncoupling these activities and studying them independently could shed insight into basic biochemical differences between transformed and primary cells that could be exploited for targeted therapeutic purposes.

Files
ExploringApoptinsAnticancerAbility043009.pdf
There is one file which has been withheld at the advisor's request.

Browse by Author | Browse by Department | Search all available E-projects

Questions? Email project-questions@wpi.edu
Maintained by webmaster@wpi.edu

Project Type	MQP
Submission date	2009-04-30
Authors	Jessie Ann Brasefield, BC Doug K Tischer, CH
URN	E-project-043009-152556
Title	Exploring Apoptin’s Anticancer Ability: Uncoupling Nuclear Export and Multimerization
Advisor	Heilman, Destin, CH
Availability	unrestricted
Abstract Apoptin, Chicken Anemia Virus VP3, selectively induces apoptosis in transformed cells but not primary cells. In all cases, Apoptin undergoes nucleocytoplasmic shuttling, but partitions into the nucleus of transformed cells and the cytoplasm of primary cells. The N-terminal NES of Apoptin and the domain for multimerization overlap and all previous attempts to uncouple these activities have failed. In this study, we successfully uncoupled nuclear export and multimerization through site-directed mutagenesis of ApI37A and ApI40A. Uncoupling these activities and studying them independently could shed insight into basic biochemical differences between transformed and primary cells that could be exploited for targeted therapeutic purposes.
Files	ExploringApoptinsAnticancerAbility043009.pdf There is one file which has been withheld at the advisor's request.