Title page for E-project-102207-015235

Project Type MQP

Submission date 2007-10-22

Author
Akanksha Sharma, BIO

URN E-project-102207-015235

Sponsor Dr. Joseph C. Holt

Title LOCALIZATION OF NICOTINIC RECEPTORS ON HAIR CELLS AND AFFERENTS OF THE TURTLE SEMICIRCULAR CANAL

Advisor
Rulfs, Jill, BC

Availability unrestricted

Abstract
A distinct alpha9 nicotinic acetylcholine receptor (nAChR) may mediate the inhibition of type II hair cells in the turtle posterior crista. This project aims to localize these nAChRs in the hair cell layer with the use of commercial antibodies and fluorophore-conjugated nicotinic antagonists (e.g., alpha-bungarotoxin). An additional goal has been the optimization and development of protocols for this specific purpose. Identification and visualization of these nAChRs will provide greater insight into the complex neural pharmacology of the inner ear.

Files
Akanksha_Sharma_MQP_Oct07.pdf

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Project Type	MQP
Submission date	2007-10-22
Author	Akanksha Sharma, BIO
URN	E-project-102207-015235
Sponsor	Dr. Joseph C. Holt
Title	LOCALIZATION OF NICOTINIC RECEPTORS ON HAIR CELLS AND AFFERENTS OF THE TURTLE SEMICIRCULAR CANAL
Advisor	Rulfs, Jill, BC
Availability	unrestricted
Abstract A distinct alpha9 nicotinic acetylcholine receptor (nAChR) may mediate the inhibition of type II hair cells in the turtle posterior crista. This project aims to localize these nAChRs in the hair cell layer with the use of commercial antibodies and fluorophore-conjugated nicotinic antagonists (e.g., alpha-bungarotoxin). An additional goal has been the optimization and development of protocols for this specific purpose. Identification and visualization of these nAChRs will provide greater insight into the complex neural pharmacology of the inner ear.
Files	Akanksha_Sharma_MQP_Oct07.pdf