Title page for E-project-121404-130336

Project Type MQP

Submission date 2004-12-14

Authors
Katie Ellen Christopher, BC
Gregory A Krane, BIO

URN E-project-121404-130336

Title Translocation of Hepatocellular Mrp2 to the Canalicular Membrane Via Activation of PKC-delta by cAMP

Advisor
Adams, David S., BB

Availability unrestricted

Abstract
Mrp2 is an organic anion and bile salt export pump that must be translocated from the cytoplasm to the hepatocyte tubule canalicular membrane to transport its substrate. Although cAMP has been shown to induce both Mrp2 translocation and PKC-delta activation, the interdependency of those two events has not been established. Hepatocytes were treated with a cAMP analogue and/or bistratene A (a known activator of PKC-delta) at varying concentrations, followed by biotinylation of cell surface protein, preparation of cell lysates, immunoprecipitation by streptavidin, and western analysis of Mrp2 levels. Like cAMP, bistratene A also induced Mrp2 translocation, and the combined effects were not additive, indicating that cAMP translocates Mrp2 by activating PKC-delta.

Files
gkkcmqp.pdf

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Project Type	MQP
Submission date	2004-12-14
Authors	Katie Ellen Christopher, BC Gregory A Krane, BIO
URN	E-project-121404-130336
Title	Translocation of Hepatocellular Mrp2 to the Canalicular Membrane Via Activation of PKC-delta by cAMP
Advisor	Adams, David S., BB
Availability	unrestricted
Abstract Mrp2 is an organic anion and bile salt export pump that must be translocated from the cytoplasm to the hepatocyte tubule canalicular membrane to transport its substrate. Although cAMP has been shown to induce both Mrp2 translocation and PKC-delta activation, the interdependency of those two events has not been established. Hepatocytes were treated with a cAMP analogue and/or bistratene A (a known activator of PKC-delta) at varying concentrations, followed by biotinylation of cell surface protein, preparation of cell lysates, immunoprecipitation by streptavidin, and western analysis of Mrp2 levels. Like cAMP, bistratene A also induced Mrp2 translocation, and the combined effects were not additive, indicating that cAMP translocates Mrp2 by activating PKC-delta.
Files	gkkcmqp.pdf