Project Type MQP Submission date 2008-12-18 Author Brad M Kaufman, BC URN E-project-121808-174409 Title Mechanisms of Cu+ Delivery From Archaeoglobus fulgidus CopZ to CopA Advisor Arguello, Jose' M, CH Availability unrestricted Abstract
Cu+-ATPases are involved in efflux of Cu+ from the cytoplasm. In vivo, these ATPases receive metal from a specific chaperone. We hypothesize the specificity of the interaction is provided by electrostatic interactions between the transporter and its chaperone. If there are polar amino acids that lie around the Cu+ binding residues of the chaperone, they could potentially interact with the transporter during Cu+ delivery. I used a model system with a Cu+ chaperone (Archaeoglobus fulgidus CopZ) and analyzed the effects on the ATPase activity of its respective transporter (CopA). I made mutants in CopZ that replaced polar residues (Asp, Ser, Thr, and Tyr) and measured its effect on the activation of CopA. There appears to be no effect on activity when the Asp is replaced with an Ala or a Lys.
Files B_Kaufman_MQP_2008.pdf
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