Document Type thesis Author Name Romagnano, Joseph F. Email Address joe at joeswebhut.net URN etd-0121104-221651 Title Aeration and Mode of Nutrient Delivery Affects Growth Of Peas in a Controlled Environment Degree MS Department Biology & Biotechnology Advisors Dr. Pamela J. Weathers, Advisor Dr. Ronald Cheetham, Committee Member Dr. John Sullivan, Committee Member Dr. Jill Rulfs, Department Head Keywords advanced life support ethylene carbon dioxide pisum sativum root hypoxia oxygen bioregenerative life support Date of Presentation/Defense 2003-12-16 Availability unrestricted
The development of a plant growth chamber capable of sustaining plant growth over multiple generations is a necessary step towards the attainment of a Controlled Ecological Life Support System (CELSS). The studies herein examine the effects of aeration abilities and rates on plants grown in three model nutrient delivery systems during germination and over the life-cycle of the plant. These studies are the first time a porous tube nutrient delivery system was compared to another active nutrient mist delivery system. During germination an indicator of hypoxic stress, alcohol dehydrogenase (ADH) activity, was measured and was more affected by aeration rate than mode of nutrient delivery. Over the life-cycle of the plant, however, plants grown in the porous tube system had the least ADH activity and the highest levels of shoot (leaf + stem), root and leaf biomass. None of the plants in any system, however, produced viable seed. This study highlights the need to optimize aeration capabilities in the root zone of enclosed chambers.
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