Document Type thesis Author Name Griffin, Jennifer Shoener Email Address jensgriffin at gmail.com URN etd-031509-151117 Title Torque Teno Virus: A Potential Indicator of Enteric Viruses Degree MS Department Civil & Environmental Engineering Advisors Jeanine Plummer, Advisor Sharon Long, Co-Advisor James OShaughnessy, Co-Advisor Keywords cell culture PCR coliphage coliform fecal indicator enteric virus waterborne disease outbreak TTV torque teno virus Date of Presentation/Defense 2009-05-01 Availability unrestricted
To protect public health, drinking water systems are monitored for indicator organisms that correlate with fecal contamination and suggest the presence of human pathogens. Total coliforms, fecal coliforms, and E. coli are the most commonly used indicator organisms. These bacteria generally colocate with fecal pollution, but some limitations exist. In particular, the ability of indicator bacteria to predict the presence of enteric viruses is questionable because of distinct transport and survival characteristics of bacteria and viruses. Although viral indicators of enteric viruses have been proposed, none have been implemented into the current regulatory framework. In this thesis, the correlation of bacteria and viruses in drinking water sources and treatment systems is reviewed, and the potential of Torque Teno virus (TTV) to qualify as an indicator virus is discussed. TTV is unique among enteric viruses as it infects approximately 80% of healthy individuals worldwide, is transmitted by the fecal-oral route, causes no observable illness, and lacks seasonal fluctuations.
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