Worcester Polytechnic Institute Electronic Theses and Dissertations Collection

Title page for ETD etd-0428103-102059

Document Typethesis
Author NameBorrelli, Alexander P
TitleSynthetic Genes for Antimicrobial Peptides
DepartmentBiology & Biotechnology
  • Joseph Bagshaw, Advisor
  • David Adams, Committee Member
  • Jill Rulfs, Committee Member
  • Keywords
  • antimicrobial peptides
  • protegrins
  • synthetic genes
  • Date of Presentation/Defense2003-04-17
    Availability unrestricted


    The goal of this project was to clone and express the antimicrobial peptide protegrin 1 (PG-1). Initially a yeast system was chosen but was discarded due to technical difficulties. Invitrogen’s bacterial T7 expression system was chosen next to express the peptide. PG-1 expression was verified by anti-his immunoblot and then the peptide was purified by IMAC. Its activity was verified using a Bacillus subtillis radial diffusion assay.

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