Document Type thesis Author Name Borrelli, Alexander P URN etd-0428103-102059 Title Synthetic Genes for Antimicrobial Peptides Degree MS Department Biology & Biotechnology Advisors Joseph Bagshaw, Advisor David Adams, Committee Member Jill Rulfs, Committee Member Keywords antimicrobial peptides protegrins synthetic genes Date of Presentation/Defense 2003-04-17 Availability unrestricted
The goal of this project was to clone and express the antimicrobial peptide protegrin 1 (PG-1). Initially a yeast system was chosen but was discarded due to technical difficulties. Invitrogen’s bacterial T7 expression system was chosen next to express the peptide. PG-1 expression was verified by anti-his immunoblot and then the peptide was purified by IMAC. Its activity was verified using a Bacillus subtillis radial diffusion assay.
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