Title page for ETD etd-0428103-102720

Document Type	thesis
Author Name	Vaitkunas, Katrina Emilee
URN	etd-0428103-102720
Title	The Genetics of TCV Resistance
Degree	MS
Department	Chemistry & Biochemistry
Advisors	Kristin K. Wobbe, Advisor
Keywords	Plant resistance Arabidopsis thaliana Turnip crinkle virus
Date of Presentation/Defense	2003-04-28
Availability	unrestricted
Abstract Most plants are capable of mounting resistance responses to various pathogen attacks. For a hypersensitive response (HR) to occur, a dominant or semi-dominant resistance (R) plant gene is required to recognize a dominant avirulence (Avr) factor of the pathogen. Three types of Arabidopsis thaliana, Dijon-17 (Di-17), Dijon-3 (Di-3), and Columbia-0 (Col-0), are significant in understanding the genetics of Turnip crinkle virus (TCV) resistance. It has been shown that three genes are needed for successful resistance to TCV in A. thaliana: the dominant R gene HRT, the recessive gene rrt, and a third gene, TIP. Crosses of Di-17 and Di-3 plants, and crosses of Di-3 and Col-0 plants are being analyzed to determine the genotype of the F1 progeny. Using cleaved amplified polymorphic sequence (CAPS) markers, it is possible to determine the genotype of the progeny compared to the wild-type parents at the HRT and TIP loci. Additionally, protein analysis tools will be employed to compare the Di-3 and Di-17 TIP alleles to determine if there are any significant differences in the protein.
Files	Vaitkunas.pdf

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