Worcester Polytechnic Institute Electronic Theses and Dissertations Collection

Title page for ETD etd-0429103-084755

Document Typethesis
Author NameChristopher, Stephen James
TitlePlant-Pathogen Interactions: Turnip Crinkle Virus Suppression of the Hypersensitive Response in Arabidopsis thaliana
DepartmentChemistry & Biochemistry
  • Kristen Wobbe, Advisor
  • Keywords
  • Turnip crinkle virus
  • arabidopsis
  • thaliana
  • TCV
  • avrRpt2
  • avrRpm1
  • avrRps4
  • systemic acquired resistance
  • resistance
  • plant disease
  • susceptability
  • PR-1
  • transgenics
  • avirulence
  • virulence
  • Avr gene
  • R gene
  • pseudomonas syringae.
  • Date of Presentation/Defense2003-04-02
    Availability unrestricted


    The presence of turnip crinkle virus (TCV) in Arabidopsis thaliana plants has previously been shown to suppress the ability of these plants to produce a hypersensitive response (HR) upon inoculation with pathogens that would normally elicit this defense response. The ecotype Colombia-0 was examined using wildtype TCV and non-pathogenic strains of Pseudomonas syringae pv. glycinea Race 4 containing virulence genes avrRpt2, avrRpm1 and avrRps4. Transgenic lines of A. thaliana that express the TCV proteins p8, p9 or CP were also examined in an attempt to determine if these proteins play a role in suppression of the HR. Crosses of these transgenic lines were made in order to determine if binary combinations of these proteins were sufficient for HR suppression. In addition, assays were completed to determine if the inhibition of the HR correlated with suppression of resistance to the virulent Pseudomonas syringae pv. maculicola ES4236 avrRpt2 growth in the plant. Finally, PR-1 protein expression was inspected by visual and quantitative GUS reporter gene assays to determine if TCV also played a role in inhibition of the plants ability to develop systemic acquired resistance (SAR).

  • schristopher.pdf

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