Worcester Polytechnic Institute Electronic Theses and Dissertations Collection

Title page for ETD etd-050114-102006

Document Typethesis
Author NameFlaherty, Erin
TitleCytoplasmic Adaptor Protein MIG-10 Interacts With Abelson Target ABI-1 During Neuronal Migration In C. Elegans
DepartmentBiology & Biotechnology
  • Elizabeth Ryder, Advisor
  • Joseph Duffy, Committee Member
  • Jagan Srinivasan, Committee Member
  • Keywords
  • C. elegans
  • ABI-1
  • MIG-10
  • Neuronal Migration
  • Date of Presentation/Defense2014-05-01
    Availability unrestricted


    Cellular migration is an essential process for establishing neural connections during development. The MIG-10/RIAM/Lamellipodin signaling proteins are thought to send positional information from guidance cues to actin polymerization machinery, promoting the polarized outgrowth of axons. In C. elegans, mutations in the gene mig-10 result in the truncation of the migration of the mechanosensory neurons. Biochemical analysis demonstrates that MIG-10 interacts with abelson-interactor protein 1 (ABI-1), and therefore investigation into whether these proteins work together in the neuron to promote migration was completed.

    To demonstrate MIG-10 cell autonomy in the neuron, transgenic strains with specific expression of mig-10 were created. mig-10 mutants were rescued in the mechanosensory, anterior lateral microtubule neuron (ALM) by neuron specific expression of mig-10 but not by epithelial expression, suggesting that MIG-10 is acting cell autonomously. To determine ABI-1 cell autonomy, transgenic strains with specific neuronal expression of abi-1 were compared to the wild type strain. abi-1 mutants were rescued by neuron specific expression of abi-1 in the ALM, suggesting that ABI-1 also functions cell autonomously in the ALM during this migration. Further investigation into the MIG-10/ABI-1 relationship was done by feeding RNAi of abi-1 in a mig-10(ct41) mutant strain. The ALM migration was not more severely truncated in the double mutant, suggesting that MIG-10 and ABI-1 work in the same pathway. Taken together, this evidence supports a model where MIG-10 and ABI-1 work together autonomously within the ALM to promote migration.

  • EFlaherty.pdf

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