Title page for ETD etd-101399-180834

Document Type thesis

Author Name Vollaro, Cindy M.

URN etd-101399-180834

Title Definition of a Cytotoxic T Lymphocyte Epitope of the Sin Nombre Hantavirus G2 Glycoprotein

Degree MS

Department Biology & Biotechnology

Advisors
Dr. Alan L. Rothman, Committee Member
Dr. Francis A. Ennis, Committee Member
Dr. Ronald D. Cheetham, Department Head
Dr. Jill Rulfs, Major Advisor

Keywords
Hantavirus Pulmonary Syndrome
hantavirus
Sin Nombre
T-cell lines

Date of Presentation/Defense 1999-05-05

Availability unrestricted

Abstract
Sin Nombre virus is a hantavirus first recognized in New Mexico in 1993. This virus is responsible for causing Hantavirus Pulmonary Syndrome, an acute, life threatening illness characterized by pulmonary edema, capillary leaking, and extreme respiratory distress. CD8⁺ cytotoxic T-cell lines specific for Sin Nombre virus were isolated from the peripheral blood mononuclear cells (PBMC) of a donor (NM3) who was naturally infected with the Sin Nombre virus, and has survived hantavirus pulmonary syndrome (HPS). Cytotoxic T lymphocyte (CTL) assays showed that one of these cell lines, 10K, specifically recognizes a nine amino acid epitope, TAHGVGIIP (amino acids 664-672 of the precursor GPC protein), which is located in the G2 protein after cleavage. Another cell line, 10c27, specifically recognized an eight amino acid epitope, AHGVGIIP (amino acids 665-672 of the precursor GPC protein), located in the G2 protein after cleavage. Using polymerase chain reaction (PCR) and CTL assays, the recognition of these epitopes was shown to be restricted by the B35.01 Class 1 human leukocyte associated antigen (HLA) allele. This information will be useful in creating a vaccine for use in immunizing people against the Sin Nombre hantavirus, as well as elucidating the pathogenesis of this disease.

Files
vollaro.pdf

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Document Type	thesis
Author Name	Vollaro, Cindy M.
URN	etd-101399-180834
Title	Definition of a Cytotoxic T Lymphocyte Epitope of the Sin Nombre Hantavirus G2 Glycoprotein
Degree	MS
Department	Biology & Biotechnology
Advisors	Dr. Alan L. Rothman, Committee Member Dr. Francis A. Ennis, Committee Member Dr. Ronald D. Cheetham, Department Head Dr. Jill Rulfs, Major Advisor
Keywords	Hantavirus Pulmonary Syndrome hantavirus Sin Nombre T-cell lines
Date of Presentation/Defense	1999-05-05
Availability	unrestricted
Abstract Sin Nombre virus is a hantavirus first recognized in New Mexico in 1993. This virus is responsible for causing Hantavirus Pulmonary Syndrome, an acute, life threatening illness characterized by pulmonary edema, capillary leaking, and extreme respiratory distress. CD8⁺ cytotoxic T-cell lines specific for Sin Nombre virus were isolated from the peripheral blood mononuclear cells (PBMC) of a donor (NM3) who was naturally infected with the Sin Nombre virus, and has survived hantavirus pulmonary syndrome (HPS). Cytotoxic T lymphocyte (CTL) assays showed that one of these cell lines, 10K, specifically recognizes a nine amino acid epitope, TAHGVGIIP (amino acids 664-672 of the precursor GPC protein), which is located in the G2 protein after cleavage. Another cell line, 10c27, specifically recognized an eight amino acid epitope, AHGVGIIP (amino acids 665-672 of the precursor GPC protein), located in the G2 protein after cleavage. Using polymerase chain reaction (PCR) and CTL assays, the recognition of these epitopes was shown to be restricted by the B35.01 Class 1 human leukocyte associated antigen (HLA) allele. This information will be useful in creating a vaccine for use in immunizing people against the Sin Nombre hantavirus, as well as elucidating the pathogenesis of this disease.
Files	vollaro.pdf