One of the prevalent complications in patients with diabetes mellitus are diabetic foot ulcers (DFUs), lesions that can affect the skin and soft tissue of the foot, which when compounded with impaired wound healing, can lead to the development of chronic, infected wounds and ultimately, amputation. DFUs are a major source of morbidity and directly associated with higher rates of mortality, while also imposing major financial consequences, accounting for approximately 1/3 of total healthcare expenditures of diabetic care. Unfortunately, despite extended periods of wound management, approximately 20% of DFU patients have unhealed DFUs after one year, with a subset of these patients requiring major amputations. This project aims to design a preliminary method for skin wound regeneration within the context of DFUs through a composite device consisting of decellularized spinach scaffolds, which provides a structural framework for wound regeneration, and electrospun scaffolds, which provides a mechanism for drug elution to facilitate regeneration and revascularization. Mechanical peel-away tests demonstrate the feasibility of generating polyethylene terephthalate (PET) composite devices with fully hydrated decellularized scaffolds that do not readily delaminate. Post-fabrication treatments and its potential cytotoxic effects were examined by comparing PET scaffolds, incorporated with recombinant Fibroblast Growth Factor-basic (bFGF), treated either through ethanol sonication or vacuum baking. For both treatments, drug elution tests demonstrated a similar linear drug release profile over the course of six days, while direct contact and media exchange biocompatibility tests demonstrated minimal cytotoxicity to cultured fibroblasts. The biological effect of the composite PET-bFGF decellularized device was tested with fibroblasts by performing a wound scratch assay on the device and also examining cellular penetration into the decellularized scaffold over the course of several days.
Please contact email@example.com for a zoom link to this event