Within WPI’s Life Sciences and Bioengineering Center, the Microscopy Core provides the opportunity to image live, fixed, and fluorescently-labeled samples using one of several available core instruments. Core staff are available to provide guidance and training. The instruments are available for external use on a fee-for-service basis. Please contact us to discuss your project.

microscopy

The Microscopy Core instrumentation includes:

Leica SP5 Point Scanning Confocal Microscope:

Leica TCS SP5 Spectral Confocal

Inverted Leica DMI 6000 CS microscope base with a conventional scanhead

z-galvo stage for z-stacks and 3D-projections

Objectives: 10x dry (0.4na), 20x multi-immersion (0.7na), 40x oil (1.25na) and a 63x oil (1.4na)

Objectives are coverslip corrected for a #1.5 coverslip (approx. 170um)

4 lasers and a total of 7 laser lines provide flexibility for fluorophore excitation

Lasers: a 405 diode, an argon gas that covers laser lines 458,476,488 and 514, and a 561 diode and a 633 HeNe gas single line

Separate workstation for image analysis and post-processing with a copy of the Leica software

Zeiss Axiovert 200M Microscope:

Workhorse microscope capable of multifluorescence imaging

Filter module with filter sets for: Hoecsht/DAPI, FITC/488/GFP and Texas Red/594

Inverted microscope base

Brightfield, phase contrast and DIC optics

Zeiss AxioCam Mrm CCD camera, Zeiss Axiovision software, motorized stage

Objectives: 10x A-Plan (10x/0.25 Ph1); 20x LD Plan-NEOFLUAR (20x/0.4 Ph2 Korr); 20x Plan-NEOFLUAR (20x/0.50 DIC II); 40x EC Plan-NEOFLUAR (40x/1.3 oil infinity/0.17 DIC III); a 40x LD Plan-NEOFLUOR (40x/0.6 Ph2 Korr infinity/0-1.5); and a 63x Plan-APO CHROMAT (63x/1.4 oil DIC III infinity/0.17)

TMC Micro-g antivibration table

In addition, we have several pieces of accessory equipment for available for use:

2 Eppendorf Transfer Man NK2 micromanipulators

Burleigh Piezo

Patch-clamp electrophysiology

Sutter Micropipette Puller (Model P-87)

Narishige micro-forge MF-900

Leica Wild M3Z dissecting microscope

Wild Heerbrugg dissecting microscope