Project Type MQP Submission date 2007-12-13 Author Girija Ramapriya, BIO URN E-project-121307-113327 Sponsor Massachusetts Institute of Technology Biomaterials Title Optimization of Monosaccharide Labeling Advisor Rulfs, Jill, BC Availability unrestricted Abstract
Two monosaccharide labeling agents, 1-phenyl-3-methyl-5-pyrazolone (PMP), and 4-(3-Methyl-5-oxo-2-pyrazolin-1-yl) Benzoic Acid (PMPA), were investigated to determine monosaccharide content in palm oil mill effluent waste (POME). Methods published in the literature were optimized to increase efficiency of monosaccharide detection to suit industry needs. The results from PMP derivatization, using pH 8.0 PBS for dilution and microwave irradiation suggest this is the most efficient derivatization method tested in this project. Monosaccharides detected in POME samples were mannose, glucose and xylose.
Files Final_MQP.pdf Title_Page[1].pdf
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